Study of the influence of ascorbyl palmitate and amiodarone in the stability of unilamellar liposomes

LUCIANO A. BENEDINI; SILVIA S. ANTOLLINI; MARIA LAURA FANANI; SANTIAGO PALMA; PAULA MESSINA; PABLO SCHULZ

MOLECULAR MEMBRANE BIOLOGY

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2014 vol. 31 p. 85 - 85

miodarone (AMI) is a low water-solubility drug, which is very useful in treatment of severe cardiac disease. Its adverse effects are associated with toxicity in different tissues. Lysosomal phospholipidosis is a potential mechanism of AMI toxicity. Several antioxidants have been shown to reduce, and prevent AMI toxicity. The aim of this work is to develop and characterize Dimyristoyl phosphatidylcholine (DMPC) liposomal carriers doped with the antioxidant ascorbyl palmitate (Asc16), in order to either minimize or avoid the adverse effects produced by AMI. The employment of liposomes would avoid the use of cosolvents in AMI formulations, and Asc16 minimizes the adverse effects of AMI. To evaluate the partition and integration of AMI and Asc16 in lipid membranes, penetration studies onto DMPC monolayers were carried out. Both AMI and Asc16 showed surface activity, affecting the surface tension of the air/water interface and reaching surface pressures of 30-35 mN/m. When a phospholipid m