Selective Photoinduced Antibacterial Activity of Amoxicillin-Coated 2 Gold Nanoparticles: From One-Step Synthesis to in Vivo 3 Cytocompatibility

SILVERO MJ; ROCA DM; ARTUR DE LA VILLARMOIS, E; FOURNIER K; LANTERNA AE; PÉREZ MF; BECERRA MC; SCAIANO JC

ACS Omega

ACS Publications

Lugar: Washington; Año: 2018

hotoinduced antibacterial gold nanoparticles were developed as an alternative for the treatment of antibiotic-resistant bacteria. Thanks to the amoxicillin coating,they possess high in vivo stability, selectivity for the bacteria wall,16 a good renal clearance, and are completely nontoxic for eukaryotic cells at the bactericidal concentrations. A simple one-step synthesis of amoxi@AuNP is described at mild temperatures using the antibiotic as both reducing and stabilizing agent. Time-resolved fluorescence microscopy proved these novel nano-photosensitizers, with improved selectivity, are bactericidal but showing excellent biocompatibility toward eukaryotic cells at the same dose (1.5 μg/mL) when co-cultures are analyzed. Their stability in biological media, hemocompat25ibility, and photo-antibacterial effect against sensitive and antibiotic-resistant Staphylococcus aureus were evaluated in vitro, whereas toxicity, renal clearance, and biodistribution were studied in vivo in male