TRIAMCINOLONE-LOADED NANOCARRIERS: NOVEL STRATEGY TO PREVENT COGNITIVE AND EMOTIONAL SEQUELAE INDUCED BY TRAUMATIC BRAIN INJURYTHROUGH MODULATION OF THE OXIDATIVE STRESS MACHINERY

Córdoba

Congreso; 1º Congreso Mundial de TOXICOLOGÍA, SALUD AMBIENTAL Y EMERGENCIA EN SALUD.; 2023

Traumatic bran inury (TBI) is known to cause cognitive and emotional deficits likely linked to early neuroinflammatory processes. We have shown that TBI increases levels of protein (AOPP) and lipid (MDA) peroxidation, which persisted over a week. Despite the efforts to develop neuroprotective treatments, most pre-clinical studies did not report significant effects. To evaluate the effectiveness of triamcinolone-loaded nanocarriers (NT) in TBI-induced cognitive and emotional sequelae, administered NT at 15 minutes and 24h post-TBI in adult male Wistar rats. Animals were submitted to novel object recognition test (NOR) 7 days post-TBI and fear memory evaluation 24 h (test 1) and 6 days (test 2) after conditioning. Brain tissue homogenates were assayed for MDA, AOPP and two antioxidant enzymes: superoxide dismutase (SOD) and catalase (CAT) 7 days after TBI. Our data showed that TBI induced a significant decrease in the discrimination index of NOR at day 7 that could be prevented by NT administration. On the other hand, TBI induced a significant decrease in the % freezing only in test 2 which was prevented in animals treated with NT. Additionally, NT prevented increments in AOPP, MDA and CAT levels at 7 days after TBI. We concluded that TBI induced neuroinflammatory mediators that could affect neuronal functioning in brain structures related to the detrimental actions on recognition memory and fear memory retention, NT administration could be a therapeutic alternative for the early treatment of neuroinflammation mediated by TBI, contributing to restoration of cognitive and emotional processing by modulating OS machinery.