Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfaces

JUÁREZ, ANDREA V.; JUÁREZ, ANA V.; WILKE, NATALIA; YUDI, LIDIA M.

ELECTROCHIMICA ACTA

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2018 vol. 281 p. 611 - 611

0013-4686

he present work shows, for the first time, the use of single-particle Brownian dynamics methodology,followed by optical microscopy, coupled with electrochemistry techniques to evaluate the fluidity ofphospholipid films adsorbed at liquid/liquid interfaces. The combination of the two methods allowsobserving the movement of latex beads at the liquid/liquid interface modified by a phospholipidmonolayer, with and without polarization by linear potential scans or potential steps. In this way, theeffect of polarization on distearoylphosphatidylglycerol (DSPG), dimyristoylphosphatidylcholine (DMPC),and distearoylphosphatidylcholine (DSPC) monolayers, adsorbed at the water/1,2-dichloroethane interface,could be directly observed and diffusion coefficient values were derived. Lateral motilities of theseparticles can be correlated with the monolayer fluidity and solid character. A comparison with thebehavior of the lipids at air/water interfaces is also included.