Triglyceride Lenses at the Air−Water Interface as a Model System for Studying the Initial Stage in the Biogenesis of Lipid Droplets

B. CARUSO; WILKE, NATALIA; M.A. PERILLO

LANGMUIR

AMER CHEMICAL SOC

Lugar: Washington; Año: 2021 vol. 31 p. 10958 - 10958

0743-7463

ipid droplets (LD) are intracellular structures consisting of anapolar lipid core, composed mainly of triglycerides (TG) and steryl esters,coated by a lipid−protein mixed monolayer. The mechanisms underlying LDbiogenesis at the endoplasmic reticulum membrane are a matter of manycurrent investigations. Although models explaining the budding-off ofprotuberances of phase-segregated TG inside bilayers have been proposedrecently, the assumption of such initial blisters needs further empiricalsupport. Here, we study mixtures of egg phosphatidylcholine (EPC) and TGat the air−water interface in order to describe some physical properties andtopographic stability of TG bulk structures in contact with interfaces. Brewsterangle microscopy images revealed the appearance of microscopic collapsedstructures (CS) with highly reproducible lateral size (∼1 μm lateral radius)not varying with lateral packing changes and being highly stable at surfacepressures (π) beyond c