Recovery from chilling modulates the acyl-editing of phosphatidic acid molecular species in barley roots (Hordeum vulgare L.)

VILCHEZ, ANA CAROLINA; MICAELA PEPPINO MARGUTTI; REYNA, MATIAS; WILKE, NATALIA; ANA LAURA VILLASUSO

PLANT PHYSIOLOGY AND BIOCHEMISTRY

ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER

Año: 2021 vol. 167 p. 862 - 862

0981-9428

n plants, lipid metabolism and remodelling are key mechanisms for survival under temperature stress. The pre-sent study attempted to compare the lipid profile in barley roots both under chilling stress treatment and in thesubsequent recovery to stress. Lipids were obtained through a single-extraction method with a polar solvent mix-ture, followed by mass spectrometry analysis. The results indicate that lipid metabolism was significantly af-fected by chilling. Most of the glycerolipids analysed returned to control values during short- and long-term re-covery, whereas several representative phosphatidic acid (PA) molecular species were edited during long-termrecovery. Most of the PA molecular species that increased in the long-term had the same acyl chains as the phos-phatidylcholine (PC) species that decreased. C34:2 and C36:4 underwent the most remarkable changes. Giventhat the mechanisms underlying the acyl-editing of PC in barley roots remain elusive, we also evaluated the con-tribu