Tau regulates the localization and function of End binding proteins 1 and 3 (EB1/3) in developing neuronal cells

SAYAS CARMEN L; TORTOSA ELENA; BOLLATI FLAVIA; RAMIREZ-RIOS SACNICTE; ARNAL ISABELLE; AVILA JESUS

JOURNAL OF NEUROCHEMISTRY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2015 vol. 133 p. 653 - 653

0022-3042

he axonal microtubule-associated protein (MAP) tau, is a well-known regulator of microtubule stabilityin neurons. However, the putative interplay between tau and End binding proteins 1 and 3 (EB1/3), thecore microtubule plus-end tracking proteins (+TIPs), has not been elucidated yet. Here, we show that acrosstalk between tau and EB1/3 exists in developing neuronal cells. Tau and EBs partially colocalize atextending neurites of N1E-115 neuroblastoma cells and axons of primary hippocampal neurons, as shownby confocal immunofluorescence analyses. Tau downregulation leads to a reduction of EB1/3 cometlength, as observed in shRNA-stably depleted neuroblastoma cells and TAU-/- neurons. EB1/3 localizationdepends on the expression levels and localization of tau protein. Overexpression of tau at high levelsinduces EBs relocalization to microtubule bundles at extending neurites of N1E-115 cells. Indifferentiating primary neurons, tau is requ