Protein Kinase D Regulates Trafficking of Dendritic Membrane Proteins in Developing Neurons

MARIANO BISBAL; CECILIA CONDE; MARIBEL DONOSO; FLAVIA BOLLATI; JULIANA SESMA; SANTIAGO QUIROGA; ALBERTO DÍAZ AÑEL; VIVEK MALHOTRA; MARIA PAZ MARZOLO; ALFREDO CÁCERES

SOC NEUROSCIENCE

Lugar: Washington; Año: 2008 vol. 28 p. 9297 - 9297

In non-neuronal cells inactivation of Protein Kinase D (PKD) blocks fission of TGN transportcarriers inducing the appearance of long tubules filled with cargo. We now report on thefunction of PKD1 in neuronal protein trafficking. In cultured hippocampal pyramidal cells thetransferrin receptor (TfR) and the low-density receptor-related protein (LRP) arepredominantly transported to dendrites and excluded from axons. Expression of kinase inactivePKD1 or its depletion by RNAi treatment dramatically and selectively alter the intracellular trafficking and membrane delivery of TfR and LRP-containing vesicles, without inhibiting exit from the TGN or inducing Golgi tubulation. After PKD1 suppression, dendritic membrane proteins are mispackaged into carriers that transport VAMP2; these vesicles distributed to both axons and dendrites, but are rapidly endocytosed from dendrites and preferentially delivered to the axonal membrane. A kinase defective mu