Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella

DRESCH, RR; ZANETTI, GD; IRAZOQUI FJ; SENDRA VG; ZLOCOWSKI N; BERNARDI A; ROSA RM; BATTASTINI AM; HENRIQUES AT; VOZARI-HAMPE MM

INFORMA HEALTHCARE

Lugar: London; Año: 2013 vol. 88 p. 1 - 1

span style="FONT-FAMILY: "Arial","sans-serif"; FONT-SIZE: 12pt; mso-fareast-font-family: Calibri; mso-ansi-language: EN-US; mso-fareast-theme-font: minor-latin; mso-fareast-language: EN-US; mso-bidi-language: AR-SA" lang="en">Axinella corrugata lectin 1 (ACL-1) was purifi ed from aqueous extracts of the marine sponge, Axinella corrugata . ACL-1 strongly agglutinates native rabbit erythrocytes. The hemagglutination is inhibited by N -acetyl derivatives, particularly N , N ’ , N ” -triacetylchitotriose, N -acetyl-Dglucosamine, N -acetyl-D-mannosamine and N -acetyl-D-galactosamine. We investigated the capacity of biotinylated ACL-1 to stain several transf