Arginine degradation by arginase in mitochondria of soybean seedling cotyledons

ARIEL GOLDRAIJ; JOE POLACCO

PLANTA

Springer Verlag

Año: 2000 vol. 210 p. 652 - 652

0032-0935

font face="AdvTimes-b" size="2"> Abstract. Arginase (EC 3.5.3.1) localization was stud- ied in soybean (Glycine max L.) seedling cotyledons. Subcellular fractionation in a discontinuous Percoll gradient showed that arginase was localized in the mitochondrion. Arginine (Arg) uptake by mitochondria was demonstrated by co-sedimentation of [3H]Arg- derived label and the mitochondrial marker enzyme cytochrome c oxidase. Arginine uptake was complete in about 10 min. Since detergent but not NaCl released most label, we conclude that Arg was taken up and not