Arginase is inoperative in developing soybean embryos

ARIEL GOLDRAIJ; JOE POLACCO

PLANT PHYSIOLOGY.

American Society of Plant Biologists

Lugar: Rockville, MD, USA; Año: 1999 vol. 119 p. 297 - 297

0032-0889

b> Arginase (EC 3.5.3.1) transcript level and activity were measured in soybean (Glycine max L.) embryos from the reserve deposition stage to postgermination. Using a cDNA probe for a small soybean arginase gene family, no transcript was detected in developing embryos. However, arginase transcripts increased sharply on germination, reaching a maximum at 3 to 5 d after germination. There was low but measurable in vitro arginase specific activity in developing embryos (less than 6% of seedling maximum). During germination arginase specific activity increased in parallel with the sharply increasing arginase transcript level. Seedling arginase activity was largely localized in cotyledons. Arginase activity was assayed