In vitro inhibition of incompatible pollen tubes in Nicotiana alata involves the uncoupling of the F-actin cytoskeleton and the endomembrane trafficking system

JUAN A ROLDÁN; HERNÁN ROJAS; ARIEL GOLDRAIJ

PROTOPLASMA

SPRINGER WIEN

Lugar: Viena; Año: 2015 vol. 252 p. 63 - 63

0033-183X

!--[if gte mso 9]> In the S-RNase-based self-incompatibility system, subcellular events occurring in the apical region of incompatible pollen tubes during the pollen rejection process are poorly understood. F-actin dynamics and endomembrane trafficking are crucial for polar growth, which is temporally and spatially controlled in the tip region of pollen tubes. Thus, we developed a simple in vitro assay to study the changes in the F-actin cytoskeleton and the endomembrane system at the apical region of incompatible pollen tubes in Nicotiana alata. Growth -but not germination- of pollen tubes of Sc10-, S7