c-Fos-activated Synthesis of Nuclear Phosphatidyl Inositol-4,5-bisphosphate [PtdIns(4,5)P2] Promotes Global Transcriptional Changes

GABRIEL FERRERO; RENNER ML; GIL GERMAN; RODRIGUEZ LUCIA; CAPUTTO BL

PORTLAND PRESS LTD

Lugar: Londres; Año: 2014 vol. 1 p. 233 - 233

-Fos is a well-recognized member of the AP-1 family of transcription factors. In addition to this canonical activity, we previously showed that cytoplasmic c-Fos activates phospholipid synthesis through a mechanism independent of its genomic AP-1 activity. c-Fos associates with particular enzymes of the pathway of synthesis of lipids at the endoplasmic reticulum and increases the Vmax of the reactions without modifying the Km values. This lipid synthesis activation is associated to events of differentiation and proliferation that require high rates of membrane biogenesis. Since lipid synthesis also occurs in the nucleus, and different phospholipids have been assigned transcription regulatory functions, herein we examine if c-Fos also acts as a regulator of phospholipid synthesis in the nucleus. Furthermore, we examine if c-Fos modulates transcription through its phospholipid synthesis activator capacity. We show that nuclear-localized c-Fos associates with and activates Phosphatidyl I