Excitation/inhibition imbalance and impaired synaptic inhibition in hippocampal area CA3 of Mecp2 knockout mice.

CALFA G, LI W, RUTHERFORD M, POZZO-MILLER L

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 2015 p. 159 - 159

ett syndrome (RTT) is a neurodevelopment disorderassociated with intellectual disabilities and caused by loss-of-functionmutations in the gene encoding the transcriptional regulator Methyl-CpG-binding Protein-2 (MeCP2). Neuronal dysfunction and changes incortical excitability occur in RTT individuals and Mecp2-deficient mice,including hippocampal network hyperactivity and higher frequency ofspontaneous multiunit spikes in the CA3 cell body layer. Here, wedescribe impaired synaptic inhibition and an excitation/inhibition (E/I)imbalance in area CA3 of acute slices from symptomatic Mecp2 knockoutmale mice (referred to as Mecp2-/y). The amplitude of TTX-resistantminiature inhibitory postsynaptic currents (mIPSC) was smaller in CA3pyramidal neurons of Mecp2-/y slices than in wildtype controls, whilethe amplitude of miniature excitatory postsynaptic currents (mEPSC)was significantly larger in Mecp2-/y neurons. Con