Early Exposure to a High-Fat Diet Impacts on Hippocampal Plasticity: Implication of Microglia-Derived Exosome-like Extracellular Vesicles

VINUESA, ANGELES; BENTIVEGNA, MELISA; CALFA, GASTÓN; FILIPELLO, FABIA; POMILIO, CARLOS; BONAVENTURA, MARÍA MARTA; LUX-LANTOS, VICTORIA; MATZKIN, MARÍA EUGENIA; GREGOSA, AMAL; PRESA, JESSICA; MATTEOLI, MICHELA; BEAUQUIS, JUAN; SARAVIA, FLAVIA

MOLECULAR NEUROBIOLOGY

HUMANA PRESS INC

Año: 2018

0893-7648

dolescence is a transitional period from childhood to adulthood characterized by puberty and brain maturation involving behavioral changes and environmental vulnerability. Diet is one of the factors affecting brain health, potentially leading to long-lasting effects. Hence, we studied the impact of early exposure (P21-60) to a high-fat diet (HFD) on mouse hippocampus, analyzing inflammation, adult neurogenesis, dendritic spine plasticity, and spatial memory. Glycemia and seric pro-inflammatory IL1β were higher in HFD mice without differences on body weight. In the HFD hippocampus, neuroinflammation was evidenced by Iba1+ cells reactivity together with a higher expression of TNFα and IL1β while the neurogenic capability in the dentate gyrus was strongly reduced. We found a predominance of immature Dil-labeled dendritic spines from CA1 neurons along with diminished levels of the scaffold protein Shank2, suggesting a defective connectivity. Moreover, the HFD group exhibite