Fasting induces remodeling of the orexigenic projections from the arcuate nucleus to the hypothalamic paraventricular nucleus, in a growth hormone secretagogue receptor–dependent manner

CABRAL, AGUSTINA; FERNANDEZ, GIMENA; TOLOSA, MARÍA J.; REY MOGGIA, ÁNGELES; CALFA, GASTÓN; DE FRANCESCO, PABLO N.; PERELLO, MARIO

Molecular Metabolism

Elsevier

Lugar: Amsterdam; Año: 2020 vol. 32 p. 69 - 69

2212-8778

bjective: Arcuate nucleus (ARC) neurons producing Agouti-related peptide (AgRP) and neuropeptide Y (NPY; ARCAgRP/NPY neurons) are activatedunder energy-deficit states. ARCAgRP/NPY neurons innervate the hypothalamic paraventricular nucleus (PVH), and ARC/PVH projections arerecognized as key regulators of food intake. Plasma ghrelin levels increase under energy-deficit states and activate ARCAgRP/NPY neurons by actingon the growth hormone secretagogue receptor (GHSR). Here, we hypothesized that activation of ARCAgRP/NPY neurons in fasted mice wouldpromote morphological remodeling of the ARCAgRP/NPY/PVH projections in a GHSR-dependent manner.Methods: We performed 1) fluorescent immunohistochemistry, 2) imaging of green fluorescent protein (GFP) signal in NPY-GFP mice, and 3) DiIaxonal labeling in brains of ad libitum fed or fasted mice with pharmacological or genetic blockage of the GHSR signaling and then estimated thedensity and strength of ARC