CALFA GASTON DIEGO
Congresos y reuniones científicas
Título:
TRANSCRIPTION FACTOR NF-kB AND THE NEURAL MORPHOLOGIC CHANGES ASSOCIATED TO LEARNING AND MEMORY
Autor/es:
VERÓNICA DE LA FUENTE, GASTÓN CALFA, ARTURO ROMANO, LUCAS POZZO-MILLER
Reunión:
Congreso; XXVII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2012
Resumen:
Despite that the idea of storing of memories involv
es morphological
modifications in synapses was proposed more than 10
0 years
ago
independently by Cajal and Tanzi, it was only recen
tly that solid experimental
evidence has confirmed that learning is associated
with changes in dendritic
spine density. However, little is known about the m
olecular mechanisms
involved in these morpho
logical changes caused by behavioural learning.
Besides, the transcription factor NF
κ
B has been involved in memory
consolidation and reconsolidation in different para
digms, both in invertebrates
and vertebrates. It is also known to trigger the tr
anscriptio
n of genes with varied
functions, including those potentially involved in
morphological plasticity. We
tested if NF-
κ
B participates in changes of spine density induced
by fear
conditioning in hippocampal CA1 pyramidal neurons.
We stained mouse
hippocampal
slices by ballistic delivery of DiI for subsequent
confocal imaging
of dendritic spines. Our results show that (1)conso
lidation induces an increase
in spine density in both apical and basal dendrites
of CA1 pyramidal
neurons;(2)the increase in spine densit
y associated to fear conditioning is
prevented by intra-hippocampal infusions of NF
κ
B inhibitors; (3)the increase in
spine density caused by fear conditioning persists
after a reconsolidation
protocol; whether the enduring spine increase after
reconsolidation also
requires NF-kB signalling is still under investigat
ion.
