CALFA GASTON DIEGO
Congresos y reuniones científicas
Título:
Effect of IGF-1 Gene Therapy on the Formation of a Contextual Fear Memory Trace
Autor/es:
LEANDRO CHAMPARINI; MACARENA LORENA HERRERA; PABLO JAVIER ESPEJO; ANDREA OTAMENDI; RAMIRO GABRIEL COMAS MUTI; GASTON DIEGO CALFA; VICTOR ALEJANDRO MOLINA; CLAUDIA BEATRIZ HERENU
Reunión:
Congreso; Argentine Society for Research in Neurosciences; 2018
Resumen:
Basolateral amygdala complex plays an essential role in the
generation of an emotional state caused by an aversive experience.
Insulin growth factor like I (IGF-I) could modulate
hippocampal circuits modifying cognitive functions, and possibly,
the molecular mechanisms involved in some psychopathologies
related to traumatic memories. The main objectives
are (a) to promote and evaluate the expression of a
memory trace through IGF-I gene therapy and (b) to evaluate
if structural plastic changes in dorsal hippocampus, are
responsible for the expression of this memory trace. Adult
male Wistar rats were bilaterally infused into BLA with RAd-
DS-Red, as a control virus and RAd-IGF-I, as therapeutic
virus. Seven days later we performed a weak fear conditioning
protocol (WFCP). Freezing behavior (FB) was assessed
as a measure of retrieval and memory retention. At Day 15,
we performed hot plate test to evaluate sensitivity damage.
Rats were perfused and the brain fixed for dendritic spine
analysis. A significantly increase in FB in the RAd-IGF-1
group was observed after 7 days and maintained for 14
days postinjection. There was not sensitivity damage in
both groups. Preliminary results for dendritic spine analysis
indicate no significant differences in spine density. IGF-I gene
therapy induces a significant expression of FB in a WFCP,
with a possible promotor effect on the formation of a fear
memory trace which prompt us to further studies under this
experimental model.
generation of an emotional state caused by an aversive experience.
Insulin growth factor like I (IGF-I) could modulate
hippocampal circuits modifying cognitive functions, and possibly,
the molecular mechanisms involved in some psychopathologies
related to traumatic memories. The main objectives
are (a) to promote and evaluate the expression of a
memory trace through IGF-I gene therapy and (b) to evaluate
if structural plastic changes in dorsal hippocampus, are
responsible for the expression of this memory trace. Adult
male Wistar rats were bilaterally infused into BLA with RAd-
DS-Red, as a control virus and RAd-IGF-I, as therapeutic
virus. Seven days later we performed a weak fear conditioning
protocol (WFCP). Freezing behavior (FB) was assessed
as a measure of retrieval and memory retention. At Day 15,
we performed hot plate test to evaluate sensitivity damage.
Rats were perfused and the brain fixed for dendritic spine
analysis. A significantly increase in FB in the RAd-IGF-1
group was observed after 7 days and maintained for 14
days postinjection. There was not sensitivity damage in
both groups. Preliminary results for dendritic spine analysis
indicate no significant differences in spine density. IGF-I gene
therapy induces a significant expression of FB in a WFCP,
with a possible promotor effect on the formation of a fear
memory trace which prompt us to further studies under this
experimental model.
