CALFA GASTON DIEGO
Congresos y reuniones científicas
Título:
Molecular mechanisms underlying promotor effect of IGF 1 o n the formation of a fear memory trace
Autor/es:
LEANDRO GABRIEL CHAMPARINI; MACARENA LORENA HERRERA; PABLO JAVIER ESPEJO; RAMIRO GABRIEL COMAS MUTIS; GASTÓN DIEGO CALFA; VICTOR ALEJANDRO MOLINA; CLAUDIA BEATRIZ HEREÑÚ
Reunión:
Congreso; XXXIV Annual Meeting of the Argentine Society for Research in Neuroscience; 2019
Resumen:
Basolateral amygdala co
mplex (BLA) plays an essential role in the generation of
an emotional state caused by an aversive experience. Insulin like growth fa ctor I
(IGF I) could modulate hippocampal circuits modifying co gnitive functions, and
possibly, the molecular mechanisms inv olved in some psychopathologies related to
traumatic memories.
Objective To evaluate the
formation of a memory trace through BLA IGF I gene
therapy and synaptic and molecular changes in dorsal hi ppocampus (HD) and BLA
associated with the formation of this memory trace.
M&M: Adult male Wistar rats were bilaterally infused into BLA with RAd
IGF I, and
RAd Ds Red as control. 7 days later we performed a weak fear conditioning
protocol (WFCP). Freezing behavior (FB) was assessed at 7th and 14th day. HD
synaptic plasticity was assesed through dendritic spine analysis. BLA and HD were
obtained for pro tein analysis.
Results: A significantly inc
rease in FB in the RAd IGF 1 group was observed after
7 days a nd 14 days post injection. There was a significant increase i n mature
spines after RAd IGF 1 treatment. pERK/ERKt level was increased in BLA in RAd
IG F 1 group at the time WFCP was performed.
C
onclusions: IGF I gene therapy induces a significant expression of FB in a WFCP,
with a possible promotor effect on the form ation of a fear memory trace. This
behavioral expression was coincident with changes in H D dendritic plasticity. This
effect could b e partially attributed to MAPK/ERK pathway activation in BLA
mplex (BLA) plays an essential role in the generation of
an emotional state caused by an aversive experience. Insulin like growth fa ctor I
(IGF I) could modulate hippocampal circuits modifying co gnitive functions, and
possibly, the molecular mechanisms inv olved in some psychopathologies related to
traumatic memories.
Objective To evaluate the
formation of a memory trace through BLA IGF I gene
therapy and synaptic and molecular changes in dorsal hi ppocampus (HD) and BLA
associated with the formation of this memory trace.
M&M: Adult male Wistar rats were bilaterally infused into BLA with RAd
IGF I, and
RAd Ds Red as control. 7 days later we performed a weak fear conditioning
protocol (WFCP). Freezing behavior (FB) was assessed at 7th and 14th day. HD
synaptic plasticity was assesed through dendritic spine analysis. BLA and HD were
obtained for pro tein analysis.
Results: A significantly inc
rease in FB in the RAd IGF 1 group was observed after
7 days a nd 14 days post injection. There was a significant increase i n mature
spines after RAd IGF 1 treatment. pERK/ERKt level was increased in BLA in RAd
IG F 1 group at the time WFCP was performed.
C
onclusions: IGF I gene therapy induces a significant expression of FB in a WFCP,
with a possible promotor effect on the form ation of a fear memory trace. This
behavioral expression was coincident with changes in H D dendritic plasticity. This
effect could b e partially attributed to MAPK/ERK pathway activation in BLA
