Influence of stress in de dynamic of GluA2 subunit during fear memory destabilization/reconsolidation process

RIVA GARGIULO M; COMAS MUTIS RG; BENDER, C.L.; CALFA GD

Congreso; XXXVIII Congreso anual de la Sociedad Argentina de Investigación en Neurociencias; 2023

When a consolidated memory is retrieved, it can enter a labile state followed by a new process of stabilization dependent on protein synthesis defined as destabilization/reconsolidation. It is known that the internalization of the glutamate receptor subunit GluA2 in the hippocampus of rodents accompanied this process and plays a key role in the plasticity of such process. However, at an experimental level, it has been observed that the exposure to emotional stress prior to contextual fear conditioning, generates resistance to the destabilization/reconsolidation process. In the present study, we assessed if a stressful event influences the dynamics of expression of the GluA2 subunit during destabilization/reconsolidation process. For this, we use previously stressed and conditioned mice C57BL/6 which were reactivated one day later in the same conditioning context. The animals were sacrificed before or after retrieval, CA1 and Dentate Girus (DG) DH were obtained for WB analysis. The preliminary findings could assure that the dynamic expression of GluA2 subunit in the hippocampus is stifled because of the stress exposure.