Lack of stabilized microtubules as a result of the absence of major MAPs in CAD cells does not preclude neurite formation

BISIG, C.G.,; CHESTA, M.E.; ZAMPAR, G.G.; PURRO, S.A.; SANTANDER, V.S.; ARCE, C.A.

FEBS JOURNAL

Blackwell Pub

Lugar: London; Año: 2009 vol. 276 p. 7110 - 7110

font size="1" face="AdvPSUnv-L"> In many laboratories, the requirement of microtubule-associated proteins (MAPs) and the stabilization of microtubules for the elongation of neurites has been intensively investigated, with controversial results being obtained. We have observed that the neurite microtubules of Cath.a-differentiated (CAD) cells, a mouse brain derived cell, are highly dynamic structures, and so we analyzed several aspects of the cytoskeleton to investigate the molecular causes of this phenomenon. Microtubules and micro.laments were present in proportions similar to those found in brain tissue and were distributed similarly to those in normal neurons in culture. Neuro.laments were