Serum-induced neurite retraction in CAD cells: involvement of an ATP-actin retractile system and the lack of microtubule-associated proteins

CHESTA, M.E.; CARBAJAL, A.; ARCE, C.A.; BISIG, C. G

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2014

ultured catecholamine-differentiated cells [which lack the microtubuleassociated proteins (MAPs): MAP1B, MAP2, Tau, STOP, and Doublecortin] proliferate in the presence of fetal bovine serum, and, in its absence, cease dividing and generate processes similar to the neurites of normal neurons. The reintroduction of serum induces neurite retraction, and proliferation resumes. The neurite retraction process in catecholamine-differentiated cells was partially characterized in this study. Microtubules in the cells were found to be in a highly dynamic state, and tubulin in the microtubules consisted primarily of the tyrosinated and deacetylated isotypes. Increased levels of acetylated or D2-tubulin (which are normally absent) did not prevent serum-induced neurite retraction. Treatment of differentiated cells with lysophosphatidic acid or adenosine deaminase induced neurite retraction. Inhibition of Rho-associated protein kinase, ATP depletion and microfilament disruption each (i