PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects

NOELIA E. MONESTEROLO, AYELEN D. NIGRA, ALEXIS N. CAMPETELLI, VERÓNICA S. SANTANDER, JUAN F. RIVELLI, CARLOS A. ARCE, CESAR H. CASALE,

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 1848 p. 2813 - 2813

ur previous studies demonstrated formation of a complex between acetylated tubulin and brain plasma membrane Ca2 +-ATPase (PMCA), and the effect of the lipid environment on structure of this complex and on PMCA activity. Deformability of erythrocytes from hypertensive human subjects was reduced by an increase in membrane tubulin content. In the present study, we examined the regulation of PMCA activity by tubulin in normotensive and hypertensive erythrocytes, and the effect of exogenously added diacylglycerol (DAG) and phosphatidic acid (PA) on erythrocyte deformability. Some of the key findings were that: (i) PMCA was associated with tubulin in normotensive and hypertensive erythrocytes, (ii) PMCA enzyme activity was directly correlated with erythrocyte deformability, and (iii) when tubulin was present in the erythrocyte membrane, treatment with DAG or PA led to increased deformability and associated PMCA activity. Taken together, our findings indicate that PMCA activity is involved