Tubulin pools in human erythrocytes: altered distribution in hypertensive patients affects Na+,K+-ATPase activity

AMAIDEN, M.R.; SANTANDER, V.S; MONESTEROLO, N.E.; CAMPETELLI, A.N.; RIVELLI, J.F.; PREVITALI, G.; ARCE, C.A.; CASALE, C.H.

BIRKHAUSER VERLAG AG

Año: 2011 vol. 68 p. 1755 - 1755

font face="Times-Roman"> The presence of tubulin in human erythrocytes was demonstrated using antibodies against total á-tubulin and different tubulin isotype. Tubulin was found to be distributed within the erythrocyte normal subjets (NS) in three distinct pools: membrane, sedimentable structure and soluble fraction. Erythrocytes from hypertensive subjets (HS) has inhibited the Na+,K+-ATPase (NKA) activity. The present study show that, in erythrocytes from HS, tubulin in the membrane is increased by ~150% compared to NS. Similar increases were observed for different tubulin isotype and for acetylated tubulin/NKA complex. Treatment of erythrocytes from NS with the microtubule-s