Acetylated tubulin associates with the fifth cytoplasmic domain of Na+,K+-ATPase: possible anchorage site of microtubules to the plasma membrane

ZAMPAR, G.G; CHESTA, M.E; CARBAJAL, A; CHANADAY, N.L; DÍAZ, N.M; CASALE, C.H; ARCE, C.A.

THE BIOCHEMICAL SOCIETY

Lugar: LONDON; Año: 2009 vol. 422 p. 129 - 129

font size="2" face="Times-Roman"> We showed previously that NKA (Na+/K+-ATPase) interacts with acetylated tubulin resulting in inhibition of its catalytic activity. In the present work we determined that membrane-acetylated tubulin, in the presence of detergent, behaves as an entity of discrete molecular mass (320?400 kDa) during molecular exclusion chromatography. We also found that microtubules assembled in vitro