Participation of ABH glycoconjugates in the secretory response to escherichia coli heta-labile toxin in rabbit intestine

ESTELA M. GALVAN; GERMAN A. ROTH; CLARA G. MONFERRAN

The Journal of Infectious Diseases

IDSA

Lugar: Chicago; Año: 1999 vol. 180 p. 419 - 419

b> The ability of membrane ABH blood group?active glycoconjugates to act as receptors of the heat-labile enterotoxin of Escherichia coli (LTh) was studied in vitro and in vivo when GM1 was blocked by the cholera toxin B subunit. Rabbits were classified as AB or H based on intestinal ABH-antigenic activities. Brush border membranes from AB rabbits contained 4 times more LTh binding sites than the H ones. LTh interaction could be inhibited by lectins that recognize ABH determinants. LTh induced a similar dose-dependent secretory response in ligated ileal loops of both types of animals. Anti-AB antibodies and Ulex europaeus I lectin could significantly reduce the fluid accumulation in AB andHrabbits, respectively. LTh cause