High affinity of anti-GM1 antibodies is associated with disease onset in experimental neuropathy

COMÍN ROMINA; YUKI NOBUHIRO; LOPEZ PABLO H. H.; NORES GUSTAVO A.

JOURNAL OF NEUROSCIENCE RESEARCH

Año: 2006 vol. 84 p. 1085 - 1085

0360-4012

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 200%; tab-stops: 36.0pt; mso-hyphenate: none">High antibody affinity has been proposed as a disease determinant factor in neuropathies associated with anti-GM1 antibodies. An experimental model of Guillain-Barré syndrome, induced by immunization of rabbits with bovine brain gangliosides or GM1, was described recently (Yuki et al., 2001. Ann Neurol 49:712-720). We searched plasma from these rabbits, taken at disease onset and one or two weeks prior to onset, for the presence of high-affinity anti-GM1 IgG antibodies. Affinity was estimated by soluble antigen binding inhibition. High-affinity antibodies (binding inhibition by 10-9 M GM1) were detected at disease