Standardized flow cytometry assay for identification of human monocytic heterogeneity and LRP1 expression in monocyte subpopulations: decreased expression of this receptor in nonclassical monocytes

FERRER D; JALDIN-FINCATI JR; AMIGONE JL; CAPRA RH; COLLINO CJ; ALBETINI R; CHIABRANDO GA

CYTOMETRY PART A

WILEY-LISS, DIV JOHN WILEY & SONS INC

Lugar: New York; Año: 2014 vol. 85 p. 601 - 601

1552-4922

e present a flow cytometry assay by which human blood monocyte subpopulations -classical (CD14++CD16-), intermediate (CD14++CD16+) and nonclassical (CD14+CD16++) monocytes - can be determined. Monocytic cells were selected from CD45+ leukocyte subsets by differential staining of the low density lipoprotein receptor-related protein 1 (LRP1), which allows reducing the spill-over of natural killer cells and granulocytes into the CD16+ monocyte gate. Percentages of monocyte subpopulations established by this procedure were significantly comparable to those obtained by a well standardized flow cytometry assay based on the HLA-DR monocyte-gating strategy. We also demonstrated that LRP1 is differentially expressed at cell surface of monocyte subpopulations, being significantly lower in nonclassical monocytes than in classical and intermediate monocytes. Cell surface expression of LRP1 accounts for only 20% of the total cellular content in each monocyte subpopulation. Finally, we establis