Parameters Affecting the Adsorption of Ligands to Polyvinyl Chloride Plates in Enzymeimmunoassay

ZALAZAR FABIAN,; CHIABRANDO, GUSTAVO A; ALDAO MARIO,; VIDES MIGUEL,

JOURNAL OF IMMUNOLOGICAL METHODS

ELSEVIER

Año: 1992 vol. 152 p. 1 - 1

0022-1759

p class="abstract">In the present work, we studied the efficacy of three blocking agents (HSA, BSA and OVA) in the inhibition of non-specific binding to PVC plates. According to the inhibition data, 1% OVA was the most effective blocking agent. On the other hand, the presence of detergents in all of the blocking solutions drastically decreased the percent inhibition of the non-specific binding. Furthermore, the effect of ligand concentration on adsorption and the kinetics of ligand adsorption to PVC plates were also investigated. Ligand adsorption is a linear function of input up to a limit (around 8.70 ng/mm2) where saturation is reached. The rate of adsorption of pure human IgG to PVC plates was proportionally increased with the temperature, as shown by proportional rate constants almost 2 times faster at 37 degrees C than at 4 degrees C. These results have practical implications for investigators using PVC for immunoassays and should be taken into consideration when designing such