uPA binding increases uPAR localization to lipid rafts and modifies the receptor microdomain composition.

SAHORES MM; PRINETTI A; CHIABRANDO GA; BLASI F; SONNINO S

Elsevier

Lugar: Reino Unido; Año: 2008 vol. 1778 p. 250 - 250

font size="3" face="TimesNewRoman"> UPAR is a GPI anchored protein which is found in both lipid rafts and the more fluid regions of the plasma membrane. We have studied the role of the ligand uPA on uPAR localization and on the composition of the lipid membrane microdomains. We have analyzed the (glyco)sphingolipid environment of uPAR in detergent resistant membrane (DRM) fractions prepared by cell lysis with 1% Triton X-100 and fractionated by sucrose gradient centrifugation. The uPAR specific lipid membrane microdomain has been separated from the total DRM fraction by immunoprecipitation with an anti-uPAR specific antibody under conditions that preserve membrane integrity. Our results show that in HEK293-uPAR cells, uPAR is partially associated with DRM and this association increases after ligand bind