Low-density lipoprotein receptor related protein-1 (LRP-1) expression in a rat model of oxygen-induced retinal neovascularization

SÁNCHEZ MC; BARCELONA PF; LUNA JD; ORTIZ SG; JUAREZ PC; RIERA CM; CHIABRANDO GA

EXPERIMENTAL EYE RESEARCH

ELSEVIER

Año: 2006 vol. 83 p. 1378 - 1378

0014-4835

p class="abstract">The low-density lipoprotein receptor-related protein-1 (LRP-1) is a high-molecular weight receptor of the LDL receptor gene family. Its ability to bind and internalize both proteinases and proteinase-inhibitor complexes from the extracellular space suggests that it has a major role in modulating uncontrolled retinal cell proliferation. In order to test this assumption, we investigated the expression of LRP-1 and receptor-associated ligands in a rat model of oxygen-induced retinal neovascularization. Wistar albino rats were placed into incubators at birth and exposed to an atmosphere alternating between 50% and 10% of oxygen every 24 h. After 14 days, the animals were allowed to recover in room air and sacrificed at postnatal day 20 (P20). The protein expression of LRP-1 and alpha2-macroglobulin (alpha2M) in the retina from unexposed and hyperoxia-exposed rats was investigated by Western blot. The localization of LRP-1 after neovascularization was assessed by immunoh