CHIABRANDO GUSTAVO ALBERTO
Congresos y reuniones científicas
Título:
Quercetin inhibitory effect on metalloproteinases activity and synthesis.
Autor/es:
SARAGUSTI A; ORTEGA G; CABRERA JL; CHIABRANDO GA
Lugar:
Córdoba
Reunión:
Congreso; XXXVIII Reunion Anual de la Asociacion Argentina de Farmacología Experimental.; 2006
Institución organizadora:
Sociedad Argentina de Farmacología Experimental
Resumen:
Flavonoids are phenolic plant constituents with a broad range of biological activities. Quercetin is the major dietary flavonoid found in fruits and vegetables. It has anti-inflammatory, anti-oxidant and anti-tumoral properties but the mechanisms through which quercetin exerts their biological actions are not fully understood. Metalloproteinases (MMPs) are endopeptidases involved in tissue remodeling by degradation of extracellular matrix (ECM). MMP-9 plays an important role in cell migration and extravasation. The activity and synthesis of MMP-9 are regulated at several levels and its production can be induced by growth factors. The exacerbation of MMP-9 activity and synthesis leads to an untimely and accelerated turnover of ECM, which is observed in pathological conditions such as acute and chronic inflammation, vascular disease and cancer. Thus, inhibition of MMP-9 activity and synthesis may conduce to an alternative therapy in these pathologies. In the present study we investigate the inhibitory effect of quercetin on MMP-9 proteolytic activity using zymographic assays. Quercetin inhibits pro-MMP-9 and active MMP-9 activity in a dose and time dependent manner. We also evaluate the effect of quercetin in the induction of MMP-9 production showing that quecetin blocks MMP-9 synthesis. Our results demonstrate that quercetin inhibits MMP-9 activity and synthesis. Thus, quercetin constitutes a potential therapeutic agent with anti-inflammatory and anti-tumoral properties.
