Insulin effect on gelatinases activity in different cell lines.

SÁNCHEZ MARÍA CECILIA; SARAGUSTI ALEJANDRA; CHIABRANDO GUSTAVO

Iguazu

Congreso; XL Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB). (2004).; 2004

Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB)

INSULIN EFFECT ON GELATINASES ACTIVITY IN DIFFERENT CELL LINES. Sanchez C, Saragusti A, and Chiabrando G. CIBICI (CONICET)-Departamento de Bioquímica Clínica. Facultad de Ciencias Químicas. UNC. The main biological role of insulin is to control the glucose level but this hormone also can regulate the cellular growth and differentiation by binding to its specific cell surface receptor (IR) which down-stream activate multiple signaling pathways. Matrix metalloproteinases (MMPs) are a family of proteases with diverse substrate specificity and their primary targets are extracellular matrix components. The gelatinases, MMP-2 and MMP-9, are a subgroup of MMPs with ability to degradate native collagen type IV, the major constituent of basement membranes, and therefore they have a key role in cancer, promoting the extravasation and migration of tumor cells. In this work we investigate the activity of the gelatinases by zymography analyzing the effect of insulin at different doses (1.10 -4 to 1.0 U/ml) and times of incubation (2 to 24 hs) in the human fibrosarcoma cell line HT1080 and the chinese hamster ovary cell line CHOK1. We demonstrate that insulin stimulates the gelatinase activity in both cell lines. At short incubation times MMP-2 activity increased with high doses of insulin (1.10.1 to 1.0 U/ml), whereas at long incubation times the MMP-9 activity was maximum with low concentration of insulin (1.10-4 to 1.10-2 U/ml). In conclusion our results indicate that the insulin has different effects on gelatinases activity, which is dose and incubation time dependent in both cell lines analyzed.