Perinatal Protein Restriction Impacts Nuclear O-GalNAc Glycosylation in Cells of Liver and Brain Structures of the Rat

GARAY YC; CEJAS RB; PERONDI MC; GUTIERREZ MC; PARODI P; FERRERO AF; LARDONE RD; VALDOMERO A; CUADRA GR; IRAZOQUI FJ

JOURNAL OF NUTRITION

AMER SOC NUTRITIONAL SCIENCE

Año: 2023

0022-3166

ackgroundPost-translational modifications are key factors in the modulation of nuclear protein functions controlling cell physiology and an individual’s health.ObjectivesThis study examined the influence of protein restriction during the perinatal period on the nuclear O-N-acetylgalactosamine (O-GalNAc) glycosylation of cells from the liver and parts of the brain in the rat.MethodsPregnant Wistar rats were divided into 2 groups on day 14 of pregnancy and fed ad libitum 1 of 2 isocaloric diets containing 24% (well-fed) or 8% (protein-restricted diet) casein until the end of the experiment. Male pups were studied after weaning at 30 d of life. Animals and their organ/tissues (liver, cerebral cortex, cerebellum and hippocampus) were weighed. Cell nuclei were purified, and the presence in nucleus and cytoplasm of all factors required for the initiation of O-GalNAc glycan biosynthesis, i.e., the sugar donor (UDP-GalNAc), enzyme activity (ppGalNAc-transferase) and the glycosylation product