Time course of IgM antibodies which block anti-myelin basic protein IgG antibodies associated with development of experimental autoimmune encephalomyelitis in rabbits

LOPEZ PHH, AL DEGANO, CG MONFERRAN, FJ IRAZOQUI, GA NORES, GA ROTH

Elsevier

Año: 2001 vol. 119 p. 30 - 30

everal authors have demonstrated the presence in normal sera of antibodies that inhibit binding of a variety of autoantibodies. These inhibitory or blocking antibodies are generally considered to play a role in humoral self-tolerance. We examined sera from normal rabbits and from rabbits with experimental autoimmune encephalomyelitis (EAE) in search for antibodies capable to inhibit reactivity of autoantibodies directed to myelin basic protein (MBP). Rabbits injected with bovine myelin  in complete Freund´s adyuvant (EAE rabbits) or with adyuvant alone (control rabbits) were bled at various intervals post-injection. Sera were subjected to chromatography on a protein A-sepharose column, reatined and nonretained fractions were collected and ability of these fractions to block reactivity of affinity-purified anti-MBP IgG-antibodies was analyzed by immunoblot technique. Protein A nonretained fraction from control rabbits inhibited anti-MBP IgG reactivity to the same degree at all int