FLUOXETINE INHIBITED THE EFFECT OF GHRELIN UPON MEMORY RETENTION AND FOOD INTAKE.

CARLINI, VALERIA P AND DE BARIOGLIO, SUSANA RUBIALES

Pinamar-Buenos Aires

Congreso; X Congress of the Panamerican Association for Biochemistry and Molecular Biology (PABMB), the XLI Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology (SAIB) and the XX Annual Meeting of the Argentine Society for Neurochemistry (; 2005

FLUOXETINE INHIBITED THE EFFECT OF GHRELIN UPON MEMORY RETENTION AND FOOD INTAKE

Carlini, Valeria P and de Barioglio, Susana.

Departamento de Farmacología. Facultad de Ciencias Químicas. Universidad Nacional de Córdoba. Ciudad Universitaria, 5000-Córdoba.  Argentina.

E-mail: vcarlini@mail.fcq.unc.edu.ar

 

Ghrelin (Gr) is a new orexigenic peptide. In a previous paper we have demonstrated that the Hippocampus (Hi), participates in the central effects of Gr, particularly on food intake and memory retention. It has been demonstrated that Gr inhibited in vitro serotonin release from rat hypotalamic synaptosomes.

In the present work we analyzed the participation of serotoninergic (5HT) neurotransmition in the increased food intake and memory retention induced by Gr using pharmacological and behavioral (step down test) approaches.

We have measured short and long term memory retention (STM, LTM) and food intake (1, 2, 4 and 24 hs post injection) in animals treated with Fluoxetine (FX) administrated intraperitoneatly (ip), a selective 5HT reuptake inhibitor, prior to hippocampal Gr (0.03, 0.3, y 3.0 nmol/ml) administration.

The treatment with FX alone decreased LTM and food intake at 1 h post treatment. FX also inhibited the effect on STM and LTM induced by Gr administration and the orexigenic effect of the peptide.

In conclusion, these results suggest that the appetite stimulating activity and the increase of STM and LTM induced by Gr could be mediated probably by a decrease in the 5-HT release induced by Gr.