Cholinesterase inhibitor effects on neurotransmitters in rat cortex in vivo.

CUADRA, G., SUMMERS, K. AND GIACOBINI, E.

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS

American Society for Pharmacology an Experimental Therapeutics

Lugar: United States; Año: 1994 vol. 270 p. 277 - 277

0022-3565

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: justify; mso-pagination: widow-orphan; mso-layout-grid-align: none">A microdialysis technique was used to investigate the effect of physostigmine (PHY) and heptylphysostigmine (HEP), administered systemically or locally, on the extracellular levels of acetylcholine (ACh), norepinephrine, dopamine and 5-hydroxytryptamine in the cerebral cortex of the rat. Levels of these neurotransmitters in dialysates were assayed simultaneously with two different high pressure liquid chromatography systems. No cholinesterase inhibitor was added into the probe to increase detection of ACh after systemic administration. Cholinesterase inhibition and its relation to ACh levels were also studied. Systemic administration of two doses of Cholinesterase inhibitor [PHY (30 and 300 ug/kg)and HEP (2 and 5 mg/kg)] produced a dose-dep