Perinatal Protein Restriction Impacts Nuclear O-GalNAc Glycosylation in Cells of Liver and Brain Structures of the Rat

CUADRA G.R.

JOURNAL OF NUTRITION

AMER SOC NUTRITIONAL SCIENCE

Año: 2023 vol. 153 p. 979 - 979

0022-3166

ackground: Post-translational modifications are key factors in the modulation of nuclear protein functions controlling cell physiology and an individual?s health.Objectives: This study examined the influence of protein restriction during the perinatal period on the nuclear O-N-acetylgalactosamine (O-GalNAc) glycosylation of cells from the liver and parts of the brain in the rat.Methods: Pregnant Wistar rats were divided into 2 groups on day 14 of pregnancy and fed ad libitum 1 of 2 isocaloric diets containing 24% (well-fed) or 8% (protein-restricted diet) casein until the end of the experiment. Male pups were studied after weaning at 30 d of life. Animals and their organ/tissues (liver, cerebral cortex, cerebellum and hippocampus) were weighed. Cell nuclei were purified, and the presence in nucleus and cytoplasm of all factors required for the initiation of O-GalNAc glycan biosynthesis, i.e., the sugar donor (UDPGalNAc), enzyme activity (ppGalNAc-transferase) and the glycosylation pro