Antithrombotic in-vivo effects of quercetin-tetra-sulphate (QTS) isolated from Flaveria bidentis in an experimental thrombosis model in mice.

GUGLIELMONE, HA; AGNESE, AM; NUÑEZ-MONTOYA, S; CABRERA, JL; CUADRA G.

Berlin

Congreso; XXVI Congress of the International Society of Thrombosis and Haemostasis; 2017

International Society of Thrombosis and Haemostasis

Antithrombotic in-vivo effects of quercetin-tetra-sulphate (QTS) isolated from Flaveria bidentis in an experimental thrombosis model in mice.Background: Quercetin 3,7,3´,4´-tetrasulphate (QTS) presents important anticoagulant, antiplatelet and pro-fibrinolytic actions besides inhibitory effects over tissue factor expression in human monocytes.Aim: In order to evaluate the antithrombotic properties of QTS, this study was designed to determine the in-vivo effects of sulfated flavonoids in an experimental thrombosis model in mice.Materials and methods: QTS was purified from an alcohol/water extract (1: 1) of Flaveria bidentis leaves by using a Sephadex G10 column and water as mobile phase. One hour before the thrombotic challenge (tail vein injection of 150 μg of collagen + 1.8 μg of epinephrine), five groups of 10 C57BL6 male mice weighing 28±2 gr received different intraperitoneal (i.p.) doses of QTS (10, 25, 50 or 100 mg/kg, respectively) or i.p. aspirin (20 mg/kg) (antithrombotic agent), as protected controls. Control animals (n = 25) received i.p. vehicle in the same volume as QTS in treated mice. The number of dead or paralyzed mice was evaluated until 15 minutes after injection of the thrombogenic substance and percent protection rate (PR) was calculated by the equation [1-(dead+paralyzed) /total]x100.Results: PRs were 100, 90, 70 and 20% for 100, 50, 25 and 10 mg/Kg of QTS, respectively. In positive and negative controls, the PRs were 70 and 4%, respectively. Histological studies showed pulmonary thrombi in a dose-dependent manner.Discussion: Taken altogether, our results suggest that QTS could be a promising candidate as antithrombotic agent and that this effect could be associated with multiple effects of this compound over the blood coagulation system.