alpha-Melanocyte-stimulating hormone modulates lipopolysaccharide plus

SCIMONELLI T

JOURNAL OF NEUROIMMUNOLOGY

ELSEVIER SCIENCE BV

Año: 2010 vol. 227 p. 52 - 52

0165-5728

n a previous work we showed that the melanocortin alpha-melanocyte-stimulating hormone (α-MSH) exerts anti-inflammatory action through melanocortin 4 receptor (MC4R) in vivo in rat hypothalamus. In this work, we examined the effect of α-MSH on the expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) and their receptors in primary cultured rat hypothalamic neurons. We also investigated α-MSH's possible mechanism/s of action. α-MSH (5 μM) decreased TNF-α expression induced by 24 h administration of a combination of bacterial lipopolysaccharide (LPS, 1 μg/ml) plus interferon-γ (IFN-γ, 50 ng/ml). Expression of TNF-α and IL-1β receptors TNFR1, TNFR2 and IL-1RI, was up-regulated by LPS+ IFN-γ whereas α-MSH did not modify basal or LPS+ IFN-γ-induced-TNFRs or IL-1RI expression. Both α-MSH and LPS+ IFN-γ treatments increased CREB activation. α-MSH did not modify NF-κ