Brain ethanol-metabolizing enzymes are differentially expressed in lead-exposed animals after voluntary ethanol consumption: pharmacological approaches

MARA S. MATTALLONI; DEZA-PONZIO, ROMINA; ALBRECHT, PAULA A.; LUCIA E. FERNANDEZ-HUBEID; CANCELA LILIANA MARINA; MIRIAM B. VIRGOLINI

NEUROTOXICOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2019

0161-813X

Developmentally-lead (Pb)-exposed rats showed an enhancedvulnerability to the stimulating and motivational effects of ethanol(EtOH). This is accompanied by differential activity of the brain EtOHmetabolizing enzymes catalase (CAT) and mitochondrial aldehydedehydrogenase (ALDH2). Based on the theory that brain acetaldehydeaccumulation is associated to the reinforcing properties of EtOH, thisstudy sought to determine brain CAT and ALDH2 expression in limbic areasof control and Pb-exposed animals after voluntary EtOH intake. Thirtyfive-day-old perinatally Pb-exposed animals (220 ppm Pb) were offeredwith water or increasing EtOH solutions (2-10%) during 28 days untilpostnatal day (PND) 63. Once intake was stable, the animals wereadministered with: 1) vehicle (VEH; test days 21-24 or 21-28, ascorresponds), or 2) a CAT activity inhibitor: 3-amine 1, 2, 4-triazole(AT; 250 mg/kg intraperitoneally [i.p.], 5 hours before the last eightEtOH intake sessions -test days 21-24 and 25-28), or 3) a CA