Abnormal cytoplasmic bundles of 8-10 nm filaments in the Neurospora crassa snowflake colonial mutant contain P59Nc.

ROSA A.L,; MARIA ELENA ALVAREZ; MALDONADO C.,

Exp Mycology

Acad Press

Año: 1990 vol. 14 p. 372 - 372

ytoplasmic bundles of microfilaments accumulate in the Neurospora crassa morphological mutant snowflake. We have performed ultrastructural, immunoelectron, and immunofluorescence microscope studies of snowflake strains and we show here that these bundles of cytoplasmic microfilaments contain 8- to IO-nm-diameter tilaments and the 59-kDa polypeptide (P59Nc) described in wild-type N. crassa strains. The immunofluorescence studies showed that almost all of the snowflake bundles are abnormal in size and morphology. Polyacrylamide gel electrophoresis of proteins from total extracts, subcellular fractions, and partially purified P59Nc from snowflake strains showed that the subcellular distribution and relative amount of P59Nc are normal in the mutants. In vitro disassembly of P59Nc bundles obtained from snowflake strains appears to occur identically to that of bundles purified from wild-type N. crassa. A polypeptide of 48 kDa enriched in a subcellular fraction of the wild-type stra