The Neurospora crassa cfp promoter drives a carbon-source-dependent expression of transgenes in filamentous fungi.

TEMPORINI E.D.,; MARIA ELENA ALVAREZ; MAUTINO M.R.,; FOLCO D.H,; ROSA, A.L.

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2004 vol. 96 p. 1256 - 1256

ims: The objective of the present study was to determine the potential of promoter sequences from the cfp gene of Neurospora crassa to drive the expression of transgenes in filamentous fungi. Methods and Results: Northern blot analyses showed that the mRNA levels of cfp were rapidly modified in response to either inducing or repressing culture conditions. The hygromycin phosphotransferase (hph) and S-adenosylmethionine synthetase (eth-1) genes were fused to a minimal cfp promoter fragment (Pcfp) and used as reporter genes. These constructs were highly expressed in transformant N. crassa strains grown in media containing glucose or sucrose and repressed in media containing ethanol or ethanol plus glucose. A gene fusion of the cfp promoter to the b-glucuronidase gene (cfp-uidA) showed identical patterns of expression in the heterologous filamentous fungus Aspergillus nidulans. Conclusions: Our results show that the levels of expression of the native cfp gene, as well as reporte