Artículos
Título:
Antibodies against the plypeptide of the N. crassa 8-10 nm filaments immunodetect a 59 kDa polypeptide in specialized rat epithelial cells.
Autor/es:
MARIA ELENA ALVAREZ; ROSA A.L,; DANIOTTI J.L,; MAGGI R.,; MACCIONI H.J.F.,
Referencias:
Año: 1991 vol. 106 p. 125 - 125
Resumen:
59Nc is a polypeptide associated with bundles of cytoplasmic and nuclear filamentous structures of 8-10 nm
of diameter in Neurospora crassa cells. It is immunologically unrelated to both higher and lower eucaryotic
tubulin and actin proteins and is detected weakly by the anti IFA monoclonal antibody. We analyze here the
immunological relationship between P59Nc and intermediate filament (IF) mammalian proteins by using
anti P59Nc, anti keratin, anti vimentin and anti IFA antibodies. Anti P59Nc antibodies detected a 59 kDa
polypeptide from rat and bovine tissues which copurifies with polypeptides of the IF family. Neither P59Nc
nor the 59 kB, a rat polypeptide were recognized by anti keratin or anti vimentin antibodies. Immunostaining
of rat tongue sections with anti P59Nc antibodies showed that the 59 kDa rat polypeptide is present in the
cortical cytoplasm of suprabasal epithelial cells.
The results indicate that P59Nc shares common antigenic determinants with a still unchara