Proline dehydrogenase contributes to pathogen defence in Arabidopsis

CECCHINI NICOLAS; MONTEOLIVA M; ALVAREZ MARIA ELENA

AMER SOC PLANT BIOLOGISTS

Lugar: Rockville; Año: 2011 vol. 155 p. 1947 - 1947

-Proline (Pro) catabolism is activated in plants recovering from abiotic stresses associated with water deprivation. In this catabolic pathway, Pro is converted to glutamate by two reactions catalyzed by proline dehydrogenase (ProDH) and D1- pyrroline-5-carboxylate dehydrogenase (P5CDH), with D1-pyrroline-5-carboxylate (P5C) as the intermediate. Alternatively, under certain conditions, the P5C derived from Pro is converted back to Pro by P5C reductase, thus stimulating the Pro-P5C cycle, which may generate reactive oxygen species (ROS) as a consequence of the ProDH activity. We previously observed that Pro biosynthesis is altered in Arabidopsis (Arabidopsis thaliana) tissues that induce the hypersensitive response (HR) in response to Pseudomonas syringae. In this work, we characterized the Pro catabolic pathway and ProDH activity in this model. Induction of ProDH expression was found to be dependent on salicylic acid, and an increase in ProDH activity was detected in cells des