Features of basal and race-specific defences in photosynthetic Arabidopsis thaliana suspension cultured cell

CECCHINI N; MONTEOLIVA M; BLANCO F; HOLUIGUE L; ALVAREZ ME

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2009 vol. 10 p. 305 - 305

p class="abstract">Plant suspension cell cultures display many features of the innate immune responses observed in planta and have been extensively applied to the study of basal and race-specific defences. However, no single model including photosynthetic cultured cells has been used for the exhaustive characterization of both basal and race-specific defences to date. In this article, we report the activation of basal and race-specific defences in green cultured cells from Arabidopsis thaliana. Inoculation of cultured cells with isogenic virulent or avirulent strains of Pseudomonas syringae pv. tomato DC3000 (Pst) was used to evaluate race-specific defences. The proliferation of avirulent Pst was found to be lower than that of virulent Pst in the inoculated cultures. Extracellular pH changes, sustained oxidative burst (5-13 h post-inoculation), enhancement of salicylic acid, and massive cell death were specifically stimulated by the avirulent bacterium. Neither avirulent nor virulent