A polypeptide of 59 kDa is associated with bundles of cytoplasmic filaments in Neurospora crassa

ROSA A.L,; MARIA ELENA ALVAREZ; LAWSON D,; MACCIONI H.J.F.,

PORTLAND PRESS LTD

Año: 1990 vol. 268 p. 649 - 649

omplex arrangements of filamentous structures have been isolated from vegetative cells of the fungus Neurospora crassa. They were enriched by differential centrifugation and purified by permeation chromatography. The filamentous structures are made up of units of 8-10 nm diameter and were isolated in bundles of up to six to nine units. The main constituent of these structures is a polypeptide with an apparent molecular mass of 59 kDa (P59Nc), which represents 4-5 % of the total N. crassa proteins. The filamentous structures are cold-stable and are not affected by high-ionic-strength solutions or by the presence of 10 mM-EDTA or 1 % (w/v) Triton X-100; they were disassembled by raising the pH of the solution or by using Tris-based buffers. The disassembled form assembled into structures sedimentable at 105000 g after dialysis against the isolation buffer. The sedimentable structures were organized in the form of regular aggregates of 42-45 nm diameter. Immunoblot experiments sh