Molecular cloning of a gene (cfp) encoding the cytoplasmic filaments protein P59Nc and its genetic relationship to the snowflake locus of Neurospora crassa.

HAEDO S.,; TEMPORINI E,; MARIA ELENA ALVAREZ; MACCIONI H.J.F,; ROSA A.L,

GENETICS SOC AM

Año: 1992 vol. 131 p. 575 - 575

59Nc is a 59-kD polypeptide associated with 8-10-nm diameter cellular filaments in normal Neurospora crassa strains. Abnormally sized and shaped bundles of these structures are present in N. crassa strains carrying mutations at the locus sn (snowflake).B y using molecular cloning and restriction fragment length polymorphism (RFLP) segregation analysis strategies we show here that sn is not the genetic locus of P59Nc. Several P59Nc cDNAs were cloned from a N. crassa XGTll library after immunoscreening with specific polyclonal anti-P59Nc antibodies. Additional longer cDNAs were obtained from a N. crassa cDNA-XZAP library. When used as probes in Southern blots of total DNA from wild-type strains, multicent-2 (a multiple mutant strain), and snowflake mutants, the P59Nc cDNAs revealed comparable patterns of hybridizing bands for all of the restriction enzymes tested. Analysis of segregation of BclI and ClaI RFLPs, detected in the genomic region of the P59Nc gene (locus cfp: cellu