Phospholipase C2 Affects MAMP-Triggered Immunity by Modulating ROS Production.

D´AMBROSIO JM; COUTO D; FABRO G ; SCUFFI D; LAMATTINA L; MUNNIK T; ANDERSSON MX; ALVAREZ ME; ZIPFEL C; LAXALT AM

PLANT PHYSIOLOGY.

AMER SOC PLANT BIOLOGISTS

Lugar: Rockville; Año: 2017 vol. 175 p. 970 - 970

0032-0889

he activation of phosphoinositide-specific phospholipase C (PI-PLC) is one of the earliest responses triggered by the recognition of several microbe-associated molecular patterns (MAMPs) in plants. The Arabidopsis (Arabidopsis thaliana) PI-PLC gene family is composed of nine members. Previous studies suggested a role for PLC2 in MAMP-triggered immunity, as it is rapidly phosphorylated in vivo upon treatment with the bacterial MAMP flg22. Here, we analyzed the role of PLC2 in plant immunity using an artificial microRNA to silence PLC2 expression in Arabidopsis. We found that PLC2-silenced plants are more susceptible to the type III secretion system-deficient bacterial strain Pseudomonas syringae pv tomato (Pst) DC3000 hrcC- and to the nonadapted pea (Pisum sativum) powdery mildew Erysiphe pisi However, PLC2-silenced plants display normal susceptibility to virulent (Pst DC3000) and avirulent (Pst DC3000 AvrRPM1) P. syringae strains, conserving typical hypersensitive response features. I