Systemic IL-12 burst expands intestinal T lymphocyte subsets bearing the alpha4beta7 integrin in mice

PEDROTTI LP, BARRIOS BE, MACCIO MARETTO L, BENTO A, SENA AA, RODRIGUEZ GALAN MC, CALIXTO JB, CORREA SG

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2016 vol. 46 p. 70 - 70

he intestinal immune system is complex and displays unique anatomic and functionalcharacteristics. Numerous subsets of immune cells are located beneath the epithelialbarrier and their activity is highly regulated. Using hydrodynamic shear of IL-12 cDNAto achieve systemic expression of IL-12 we evaluated the effect of the transient burst ofthe cytokine on the activation status of T cells from Peyer`s patches (PP), mesentericlymph nodes (MLN) and colonic lamina propria (LP). Following systemic IL-12 release,intestinal T lymphocytes became activated, exhibiting a CD44high CD62L- phenotype.After 5 days of the cytokine burst, the frequency of α4β7+ CD4+ and CD8+ cellsincreased, and CD8+ α4β7+ cells mainly expressed the transcription factor T-bet, acritical regulator of the Th1 differentiation program. The increment of the homingmolecule involved the IL-12 receptor- signal transducer and activator oftranscription (STAT)-4 axis and was independent of IFNγ, IL-4, IL-1